What are the disadvantages of a light microscope
Optical Microscope (English Optical Microscope, abbreviated OM) is the use of optical principles, the human eye can not distinguish the tiny objects magnified imaging, for people to extract the microstructure of the information of optical instruments. Ordinary optical microscope can not see 1 nanometer.
In terms of application in biology, the resolution of optical microscope is far inferior to that of electron microscope, because the resolution of optical microscope is limited by the diffraction limit, so it is impossible for its resolution to be less than half of the wavelength of the incident light. That is to say, if the incident light of 400nm, then the observation object can not be less than 200nm, but because it can be real-time, dynamic observation, the status in biology is incomparable, just like in the field of biology can not leave the fluorescence microscope and confocal and other optical microscopes. And electron microscope because of the use of electron beam to scan imaging, its resolution can easily reach the nanometer level, which is irreplaceable for the application of high-resolution imaging.
What you can see with an optical microscope
1, the light microscope can see the organelles: mitochondria, chloroplasts, vesicles, nucleoli and other structures with a size of more than 0.2 microns.
2, electron microscope can see the organelles are: mitochondria, chloroplasts, Golgi, endoplasmic reticulum, centrosomes, lysosomes, vesicles, ribosomes, peroxisomes, microsomes, bacterial plasmids, mitochondria, centrosomes, Golgi, pores in the cell wall.
