Why should we wipe the cedar oil on the oil lens when using the microscope oil lens?
Because the refractive index of glass is similar to that of cedarwood oil, adding cedarwood oil during microscopy can counteract the refraction of light passing through the glass and avoid image blurring.
When using an oil lens, it is allowed not to add tar between the spotlight and the specimen, that is, air is still used as the medium on the spotlight, but this sacrifices the resolution of the objective lens.
After observing with an oil microscope, if it is necessary to turn back to the high-power objective lens for observation, the oil on the cover glass should be wiped off to avoid contaminating the high-power objective lens. But the oil on the concentrator doesn't need to be wiped off, as long as the light barrier is slightly reduced.
After using the oil mirror, it is necessary to wipe the cedarwood oil clean in a timely manner. You can use clean lens cleaning paper to wipe off most of the oil on the lens 1-2 times first.
Then wipe twice with xylene moistened wiping paper, and finally wipe clean with wiping paper. The wiping method for the spotlight is the same as this. If the specimen needs to be preserved, the cedar oil on the slide can be wiped clean using the "paper pulling method".
Cover the glass slide with wiping paper, drop a drop of xylene on the paper, and drag the paper out while it is wet. Repeat this process several times to clean it off.
The difference in usage between oil lenses and ordinary objective lenses.
When using an oil lens, a drop of tar needs to be dropped on the glass slide, and the objective lens cannot detach from the oil drop during the ascent process. Moreover, the magnification of the oil lens is larger than that of a regular objective lens.
When using oil mirrors, attention should be paid to:
1. After using the oil lens, first use a lens cleaning paper dipped in a small amount of xylene to wipe off the cedar oil on the lens and specimen, and then use dry lens cleaning paper to clean them. After adding tar, the field of view noticeably darkens. It is necessary to raise the light collector to the highest position and open the aperture to the maximum. Before using the oil mirror, the target observed by the low-power and high-power lenses must be in the center of the field of view.
2. Cypress oil is a specialized oil for oil mirrors. Dropping a liquid with a refractive index of 1.5 under a 100x oil mirror can significantly increase the resolution of the oil mirror and improve the observation effect of the microscope. Cypress oil has a refractive index of 1.52, making it an excellent oil for microscope oil mirrors.
3. When using a microscope oil mirror, the microscope must be upright on the table, and the mirror arm must not be bent, causing the stage to tilt, in order to avoid the overflow of asphalt, affecting observation, and contaminating the table surface
