8 steps to use a microscope
1. During the experiment, the microscope should be placed on the table, with the mirror holder about 6-7cm away from the edge of the table, and the bottom light source switch should be turned on.
2. Rotate the converter to align the low magnification lens with the light hole on the stage. Then gaze into the eyepiece with both eyes, adjust the intensity of the light source, raise the spotlight, adjust the rainbow aperture to the maximum, and reflect the light into the mirror. At this point, the field of view appears bright.
3. Place the piece to be observed on the stage, so that the observed part is located in the center of the light hole.
4. First, observe with a low magnification lens (objective lens 10 x, eyepiece lens 10 x). Before observation, rotate the coarse focus screw to raise the stage and gradually bring the objective lens closer to the slice. Please note that the objective lens should not touch the glass slide to prevent it from crushing the slide. And rotate the coarse focus spiral to slowly lower the stage, and soon you can see the magnified image of the material in the slide.
If the object image seen in the field of view does not meet the experimental requirements (the object image deviates from the field of view), the ruler can be slowly moved left and right. When moving, it should be noted that the direction of the slide movement is exactly opposite to the direction of the object image seen in the field of view. If the image is not very clear, you can adjust the fine focus spiral until the image is clear.
6. If further observation is carried out using a high-power objective lens, the part of the object image that needs to be magnified for observation should be moved to the center of the field of view before converting to a high-power objective lens (when converting a low-power objective lens to a high-power objective lens for observation, the range of the object image in the field of view is greatly reduced). Generally, microscopes with normal functions have low magnification and high magnification objectives that are basically in focus. When observing clearly with a low magnification objective, switching to a high magnification objective should allow the object image to be seen, but the object image may not be very clear. It can be adjusted by rotating the fine focus screw.
7. After converting to a high magnification objective and seeing the object image clearly, the aperture or condenser can be adjusted as needed to make the light meet the requirements. When switching from a low magnification objective to a high magnification objective for observation, the field of view will slightly darken, so it is necessary to adjust the strength of the light.
After observation, the objective lens should be moved away from the light hole first, and then the microscope should be restored. And check if the parts are damaged (especially pay attention to checking if the objective lens is wet, if it is wet, use lens paper to wipe it clean). After the inspection and treatment are completed, it can be put back in its original place.
