Adjustment and Acceptance Method for Polarizing Microscopes
(1) Installation of eyepiece: Place the eyepiece into the positioning slot on the lens barrel, generally using the eyepiece crosshair in the east, west, north, and south directions. If there is any deviation, it needs to be adjusted appropriately.
(2) Installation of objective lens: When installing the objective lens in a polarizing microscope, the lens barrel should be raised to a certain height to avoid damaging the lens. Hold the objective lens with your right hand and press the spring card on the lens barrel with your left hand. Place the positioning pin on the objective lens into the positioning slot of the spring card. Pay attention to proper placement, otherwise it cannot be observed.
1. Adjustment of focal length
Place the thin film in the center of the stage and clamp it with a thin film clip. Note that the cover glass of the thin film must face upwards, otherwise it cannot be focused accurately and may damage the thin film and objective lens.
Looking at the lens barrel from the side of the microscope, turn the coarse and fine screws to lower the barrel to the * low position. Then, while observing from the eyepiece, turn the coarse and fine screws to raise the barrel until the object image is basically clear. Finally, use the fine adjustment screw to adjust the objective lens until it is completely clear. How to adjust the acceptance of polarizing microscope
How to adjust and accept the calibration polarizing microscope at the center
When using a microscope, it is often necessary to rotate the animal stage. In order to avoid rotating the object image out of the field of view, it is necessary to keep the object image at the center of the field of view stationary. This requires the central axis of the eyepiece and objective lens, as well as the rotation axis of the stage, to be strictly aligned.
In practice, after the microscope is assembled, the center axis of the eyepiece and the stage are fixed, and only the objective lens needs to be frequently removed and installed. The central axis of the objective lens is prone to deviation, and generally only the position of the central axis of the objective lens needs to be corrected. The calibration of the objective lens is carried out using screws that are perpendicular to each other in two directions. In addition, there are two adjustment handles installed on the adjustment screws, making adjustment more convenient. How to adjust the acceptance of polarizing microscope.
The correction of the center is as follows:
(1) Firstly, check if the objective lens is installed correctly, otherwise it cannot be calibrated.
(2) Then install the thin film and select a small object in the field of view, carefully placing it at the intersection of the crosshairs.
(3) Rotate the stage, and if the center is already aligned, the object image will rotate around the center of the crosshair without completely leaving the center, while the other objects will move in a circular motion around the center. If the center is not aligned, the object will leave the center of the crosshair and move in a circular motion around another center, forming an eccentric circle.
