Advantages of confocal laser microscopy include
Laser confocal microscopy is a new technology that integrates laser technology, microscope technology, fluorescence technology, computer and image processing technology, precision mechanical technology, etc., as well as high, precise and sophisticated cell analysis and engineering technology. Making it a new generation of powerful research tool in the fields of morphology, molecular cell biology, neuroscience, pharmacology, genetics and other fields.
Laser confocal microscopy is the most advanced optical microscope today. Its main advantages are:
1. Using laser as the light source, after labeling with the corresponding fluorescent probe, the sample is scanned point by point, and a two-dimensional optical cross-sectional image is obtained layer by layer. It has the function of "cell CT" and can be supported by computer three-dimensional reconstruction software. Obtain three-dimensional images and rotate them at any angle to observe the three-dimensional morphology and spatial relationships of cells and tissues;
2. It can observe living cells and tissues without damage, and dynamically measure physiological information of living cells such as intracellular Ca ion concentration and pH value;
3. It can measure the fluidity of cell membranes, intercellular communication, cell fusion, cytoskeleton elasticity, etc., and can be used as a "light knife" to complete intracellular "surgery". This technology enables in-situ dynamic and quantitative observation and measurement of living cells and tissues.
The tool microscope uses the principle of optical imaging to magnify and image the object to be measured using optical lenses such as objective lenses and eyepieces, and is supplemented by precision displacement measurement components such as X-Y fine-tuning moving positioning mechanisms and optical rulers to measure various apertures and cylinder outer diameters. Determination of other mechanical dimensions. The main applications and uses of tool microscopes are projection measuring instruments and tool microscopes. This type of measuring machine that uses optical imaging principles has the following common characteristics:
1. Non-contact measurement, most suitable for measurement of easily deformed objects;
2. Using other accessory equipment, more complex-shaped objects can be easily measured;
3. The operation is simple and can measure the pore diameter and cylinder outer diameter;
4. Accessories such as fixtures, rotating tables, and X-Y worktables can be added to control and adjust the observation angle and position of the object to be measured.
The application measurement of tool microscope can include the following items: 1. Cartesian coordinate measurement; 2. Polar coordinate measurement; 3. Angle measurement; 4. Height measurement; 5. Aperture measurement; 6. Cylinder outer diameter measurement; 7. Thread measurement; 8. Measurement accuracy, etc.
