Advantages of Confocal Laser Scanning Microscopy in Biological Research
Compared with traditional optical microscopes, confocal laser scanning microscopes feature higher resolution, allow simultaneous observation of multiple fluorescence signals, and can generate clear three‑dimensional images. In the observation of biological samples, confocal laser scanning microscopy possesses the following advantages:
Continuous scanning of living tissues, cells or cell sections enables the acquisition of detailed three‑dimensional images of the cytoskeleton, chromosomes, organelles and cell membrane systems.
It provides images with higher contrast and resolution than conventional fluorescence microscopes, while offering high sensitivity and excellent protection for specimens.
Acquisition of multi‑dimensional images. It supports 7‑dimensional imaging (XYZaλIt), including XYT, XZT and XT scanning, time‑series scanning, rotational scanning, regional scanning and spectral scanning; image processing can also be performed conveniently.
Intracellular ion fluorescence labeling. Single or multiple labeling can be used to detect and dynamically monitor intracellular pH values as well as the concentration ratios and dynamic changes of ions such as sodium, calcium and magnesium.
Fluorescence labeling observation. It allows the observation of fluorescently labeled biological substances in living cells or tissue sections, including membrane markers, chemical reactions, receptors and ligands, nucleic acids and other components. Multiple substances on the same sample can be labeled and observed simultaneously.
Cell detection is non‑invasive, stable, reliable and highly repeatable. All data and images can be exported in real time or stored long‑term.
