Advantages of Laser Scanning Confocal Microscope over Optical Microscope
① The images obtained by laser scanning confocal microscope are recorded in the form of electrical signals. Therefore, various analog and digital electronic technologies can be adopted for image processing.
② The laser scanning confocal microscope uses a confocal detection system to effectively eliminate optical signal interference outside the focal point, improve resolution, and significantly enhance the breadth and depth of the field of view. It enables non-destructive optical sectioning and achieves three-dimensional spatial positioning.
③ Since the laser scanning confocal microscope can collect and record detection signals in real time, it opens up a new way to observe the fine structures of cells and track specific molecular and ionic biochemical changes for life science research.
④ In addition to imaging functions, the laser scanning confocal microscope also has image processing functions and cell biological functions.Image processing functions include optical sectioning, 3D image reconstruction, cellular physical and biological measurement, fluorescence quantification, localization analysis, and real-time quantitative measurement of ions.Cell biological functions include sorting of adherent cells, laser cell microsurgery, optical tweezers technology, fluorescence recovery after photobleaching (FRAP), etc.
