Basic Steps to Operate the Microscope and the Digital Camera
1. Warm up the fluorescent light source. (For fluorescence microscopes)
2. Without placing a glass slide, adjust the shooting rod to the digital camera for white balance.
3. Adjust the shooting lever to the eyepiece observation position.
4. Adjust the distance between the pupils
5. Adjust the disparity
6. Search for the target and focus under clear field of view and low magnification objective lens
7. Change the appropriate objective lens and focus.
8. Adjust the brightness of the light source
9. Adjust the spotlight
10. Adjust the filter or aperture as needed
When a clear image is visible in the eyepiece, adjust the microscope to the digital camera operation steps as follows:
11. Adjust the shooting lever to transfer the image to the digital camera.
12. Check if it is in focus under bright field of view. If it is not in focus, turn the fine focus adjustment knob to focus. At this point, it should be a clear field of view, keeping the camera in a high frame rate state.
13. Adjust the color of the camera by manually balancing white or adjusting the gain values of red, green, and blue as needed.
14. Take photos,
When shooting, in bright field of view, the CMOS camera should lower the gain value and adjust the saturation as needed.
When taking fluorescent photos, it is recommended to choose the appropriate gain value and lower the camera frame rate to ensure low noise in the photos.
If you need to take good fluorescent images, the camera should be optimized for microscopic photography, capable of cooling and reducing noise. In addition, the camera has a good frame rate that can be adjusted to reduce noise.
