Biological Application-Laser Confocal Microscopy
1. Applicable to almost all cell research fields such as cell biology, cell physiology, neurobiology and neurophysiology.
2. Non-destructive real-time observation and analysis of living cells, and research on the combination of morphology and function. No damage to cell detection, safe, reliable and excellent repeatability; data images can be output in time or stored for a long time.
3. Continuous tomographic scanning of living cells and tissues or cell tissue slices can obtain fine individual cells or a group of cells or the various levels of structure (two-dimensional and three-dimensional) of the observed local tissue (including cell-specific structures-such as cytoskeleton , chromosomes, organelles and cell membrane systems, the deep structure of the sample) and complete three-dimensional images (such as analyzing changes over time, that is, four-dimensional images, and images that change with fluorescence wavelengths can also be performed to achieve more dimensional images ). **Locate the spatial position of tissue cells and other object structures that need to be observed, and perform real-time dynamic observation, analysis and recording; analysis of qualitative, quantitative, timing and positioning distribution.
4. Observation of living cells or sliced specimens of biological substances, membrane labeling, cell tracking, substances, reactions, receptors or ligands, nucleic acids, etc., labeled with fluorescent labeling probes; simultaneous multiple substance labeling can be performed on the same sample , while observing.
5. Fluorescent labeling of intracellular ions, single labeling or multiple labeling, detection of ratio determination and dynamic changes of intracellular pH and sodium, potassium, calcium, magnesium and other ion concentrations;
6. Measurement of cell membrane potential, detection of free radicals, etc.;
7. Carry out precise positioning fluorescence bleaching recovery experiments, combined with fluorescence loss experiments in fluorescence bleaching, to study intercellular communication and other related intracellular substances (molecules, etc.) movement; in time scanning experiments and photobleaching (photoquenching) experiments, The data and images of each channel can be simultaneously output and converted. Fluorescence resonance energy transfer experiments are carried out to study the movement and interaction of molecules and ions in cells through the change of fluorescence wavelength.
8. It is very accurate (spatial positioning, quantification, fixed wavelength, fixed time), sensitive, fast and can complete multiple fluorescent labeling of cell tissue at the same time (even if the emission wavelength is very close, such as multiple fluorescence with a difference of only a few nm) ) separation and observation analysis of various wavelength images, as well as online measurement and analysis functions such as colocalization of multiple fluorescent labels
