Biological Applications - Laser Microscopy
1. Applicable to cell biology, cell physiology, neurobiology and neurophysiology and almost all other fields involving cell research.
2. Non-destructive real-time observation and analysis of living cells, and combined morphological and functional studies. Non-invasive, reliable and reproducible cell detection; data images can be output in time or stored for a long time.
3. Continuous tomography of live cells and tissues or cellular tissue sections, can obtain a fine single cell or a group of cells or the observed local tissue structure at all levels (two-dimensional and three-dimensional) (including cell-specific structures - such as the cytoskeleton, chromosomes, organelles and cell membrane system, the sample of the deeper structure) and the complete three-dimensional image (such as the analysis of changes over time), i.e., four-dimensional image, but also to analyse the changes over time. (e.g., analysing changes over time, i.e., four-dimensional images, but also changes over fluorescence wavelengths, which allows for more multi-dimensional images). Locate the spatial position of tissue cells and other object structures that need to be observed for real-time dynamic observation, analysis and recording; ** analysis of qualitative, quantitative, timing and positioning distribution.
4. Fluorescent labelling probes labelled with cellular biomaterials of living cells or sectioned specimens, membrane labelling, cellular tracers,
Substances, reactions, receptors or ligands, nucleic acids, etc. Observation; simultaneous multiple substance labelling and simultaneous observation can be performed on the same sample.
5. Fluorescent labelling of intracellular ions, single or multiple labelling, detection of intracellular such as pH and sodium, potassium, calcium and magnesium.
6. Measurement of cell membrane potential, detection of free radicals, etc;
7. Perform **positioned fluorescence bleaching recovery experiments, combined with fluorescence loss in fluorescence bleaching experiments, the study of intercellular communication and other relevant intracellular substances (molecules, etc.) movement; in the time-scanning experiments and photo-bleaching (light quenching) experiments, can be simultaneous simultaneous output and conversion of the data and images for each channel. Perform fluorescence resonance energy transfer experiments to study the movement of molecules and ions within the cell through the change of fluorescence wavelength and interaction.
8.Very (spatial positioning, quantification, fixed wavelength, fixed time), sensitive, fast and can be completed at the same time on the cell tissue of multiple fluorescent markers (even emission wavelengths are very close, such as the difference of only a few nm multiple fluorescence) of various wavelengths of the image separation and observation and analysis, as well as multiple fluorescent markers co-localisation of the on-line measurement and analysis functions.
