Biological microscope calibration method
The calibration method of biological microscope should include the following four aspects.
1. Appearance
The imaging of the optical system in the entire field of view should be clear, and there should be no shadows or reflected light spots that affect the observation in the field of view. The clear area of imaging should be concentric with the field of view. The movement of each part should be flexible and stable, without sticking and jumping, and the illumination in the field of view should be uniform.
2. Fine-tuning the handwheel scribe line interval
The fine-tuning handwheel should be flexible and stable, with moderate tightness. For fine-tuning the interval between the marking lines of the handwheel, measure the starting, middle and end positions of the handwheel. Before measurement, fix the indicator on the biological microscope with a special fixture to ensure that the indicator and the microscope are fastened and firm. When fixing the indicator, ensure that the measuring head is in vertical contact with the stage. Adjust the focusing handwheel of the biological microscope to zero the reading of the indicator. Rotate the focusing handwheel 10 scales, read the indicated value, and take 1/10 of the indicated value as the measurement result. The average value of the measurements at the start, middle and end positions is used as the calibration result.
3. Objective lens magnification error adjustment
Adjust the light source to an appropriate position, install the eyepiece with a graduated reticle on one of the sleeves of the biological microscope, place the standard glass scale on the stage, and adjust the eyepiece and the standard glass so that the reticle and the standard The scale lines of the glass ruler are parallel to avoid measurement errors. Adjust the stage so that the zero position of the glass ruler is aligned with one end of the reticle, and then read directly.
4. Adjustment of reticle interval error of eyepiece ruler
After calibrating the magnification error of the biological microscope objective, this project can be without metrological calibration. The specific calibration method is to place a standard glass scale with a division value of 0.01mm on the stage, install a 10x microscope eyepiece, and focus until the eyepiece has a clear field of view. Adjust the microscope eyepiece and the standard glass scale to align the end lines of the two, and ensure that the lines and scales of the two are parallel.
