Classification of Polarizing Microscopes
1. Binocular polarizing microscope
2. Computer polarized microscope
3. Digital polarizing microscope
4. Transreflective polarizing microscope
The application of polarizing microscope
In living organisms, different types of fibrin, such as collagen, elastic fibers, tension fibers, muscle fibers, etc., exhibit significant anisotropy in their structures. Before measurement, detailed information on the molecular arrangement in these fibers can be obtained using a polarizing microscope.
2. Polarizing microscopy can be used to observe biological materials with crystal structures, such as starch granules.
3. Study living cells of animals and plants, such as spindle fibers in plant cells.
Equipment requirements for polarizing microscopes
a. Light source: It is best to use monochromatic light, and light sources can be used for general mirror inspection.
b. Eyepiece: A cross shaped eyepiece is required.
c. Focusing mirror: In order to achieve parallel polarization, a shake out focusing mirror that can push out the upper lens should be used.
d. Bertrand lens: an auxiliary component in the optical path of a spotlight, which amplifies all primary phases caused by an object into secondary phases. It ensures that the focal plane behind the objective is observed using an eyepiece
The flat crossing pattern formed in the surface.
Precautions for using a polarizing microscope
1. Do not loosen all fixing screws (except for the platform brake screws).
2. The lower polarizer is prone to detachment and should not be rotated frequently.
3. Before placing the thin film, slightly lower the stage with the cover glass facing upwards and avoid touching the objective lens. Do not apply pressure to the stage when moving thin sheets.
4. Do not place the thin film on other objects to avoid dragging and damaging it. Precautions for the use and maintenance of microscopes
When replacing the objective lens, rotate the objective lens turntable and do not pull the objective lens.
6. When using polarizing lenses, test plates, and Bourgeois lenses, they should be gently pulled and delivered.
7. If the microscope is not used for a long time, turn off the power switch on the mirror holder or adjust the brightness to the minimum.
