Debugging methods commonly used in Olympus microscopes
1. Adjustment of Olympus Microscope Illumination Optical Path System
In order to make the field of view of the Olympus microscope receive uniform and sufficient illumination, the illumination light path system must be adjusted when the Olympus microscope is installed and commissioned for the first time. This is the correct use of the Olympus microscope, and It is an important means and the most basic requirement to obtain correct and accurate results. In addition, correctly mastering the adjustment of the illumination optical path system is a necessary step after replacing the light source bulb in the process of using an Olympus microscope, and it is also a necessary step in the daily use process. The necessary means to test the performance of the Olympus microscope from time to time. The adjustment of the Olympus microscope illumination optical path system mainly includes the following 4 items:
(1) Preliminary adjustment of the light source chamber outside the microscope
① First, open the shell of the lamp room, and put the halogen bulb into the socket by pressing the spring clip. When installing, avoid direct contact with the bulb with your fingers (it can be separated by a soft cloth or paper), so as to avoid fingerprints and other dirt on the bulb, which will affect the bulb. service life.
② Put the lamp room on the table, after turning on the power, use a special screwdriver to adjust the focusing knob hole of the lamp (marked with "←→"), so that the filament is projected on the wall 1-2m away, and the filament image is adjusted. Then adjust the height of the lamp to adjust the filament hole (marked with "──") to make the filament position appropriate; then adjust the left and right position of the lamp to adjust the screw hole (marked with "──") to make the filament in the right position. .
(2) The purpose of checking and calibrating the position of the light source illuminator (filament) in the Olympus microscope is to adjust the image end of the illuminator into the field of view of the objective lens, so as to ensure that Olympus The field of view of the Bass microscope is fully and uniformly illuminated, which is a prerequisite for adjusting the Kühler illumination system. Basic tools required: Centering telescopes are equipped when purchasing an Olympus microscope.
① Unplug the frosted glass sleeve in the lamp house and put the lamp house back on the Olympus microscope;
② Select the 10× objective lens, turn on the light source program to find the sample and adjust the focus clearly, and then use the 40× objective lens to focus the sample clearly (the 40× objective lens can clearly see the whole picture of the filament);
③ Open the aperture diaphragm and field diaphragm of the condenser to the maximum;
④ Unplug one of the eyepieces, put on the centering telescope, grab the white part, and extend the black eyepiece with the other hand, and you can see the filament image in the field of vision;
⑤ If the filament position is not suitable, adjust the "──" hole, adjust the filament image in the horizontal direction, adjust the "──" hole, adjust the filament image in the vertical direction, until the filament image is adjusted to just fill the aperture of the objective lens. light circle;
⑥ After the adjustment is completed, insert the frosted glass sleeve back to its original position, unplug the centering telescope, and replace it with the eyepiece for the next adjustment. The calibration of the position in the Olympus microscope only needs to be done when the Olympus microscope is installed and debugged for the first time and when the bulb is replaced. Return the above steps to the original state.
(3) Correct adjustment of the Kohler illumination (Kohler) system Correct adjustment of the Olympus microscope, one of the main tasks is the adjustment of the illumination light path system, and the key is the adjustment of the Kohler illumination system. Olympus microscope personnel, especially those who do photomicrography, should have a certain understanding and mastery of the principle of the Kuehler illumination system and its adjustment steps, in order to give full play to the Olympus microscope. Function, the photos taken can be consistent and perfect in effect. The principle of the Kühler illumination system is simply: the light emitted by any point on the light source can illuminate the field of view of the Olympus microscope, while The light emitted by each point on the light source illuminator is collected to achieve a very sufficient and uniform illumination in the field of view of the Olympus microscope. The purpose of adjusting the Kühler illumination system is to make the observed field of view It can obtain uniform and sufficient illumination, and prevent stray light from affecting or interfering with the imaging system, so as to avoid the formation of fog on the negative during photography. The necessary components of the high-adjustment Kühler illumination system: field diaphragm, can be adjusted on-axis condenser system.
① Select 10× objective lens and 10× eyepiece;
② Put the front lens of the condenser into the optical path, adjust the aperture diaphragm to a moderate position (not too big or not too small), then raise the condenser to the top position, and adjust the condenser dial to the bright field "J" position;
③ Adjust the field diaphragm to the smallest (0.1);
④ Put the sealed biological sample on the stage, turn on the light source, and focus clearly;
⑤ A locally illuminated area or bright spot will appear in the field of view, which is the blurred image of the field diaphragm, in which the details of the sample can be clearly seen; See the details of the sample clearly;
⑥ Slightly lower the condenser to make the bright spot in the field of view gradually narrow and gradually become a clear polygonal image, which is the clear image of the field diaphragm;
7. Under normal circumstances, the polygonal image is not in the center of the field of view. It is necessary to adjust a pair of centering screws of the condenser to adjust the polygonal image of the field of view to the center position;
⑧ Gradually open the field of view diaphragm to make the polygonal image become the inscribed polygon of the field of view, and further check the alignment condition. If the alignment is not ideal, continue to adjust the alignment screw slightly;
⑨ Slightly open the field of view diaphragm slightly, so that the polygonal image just disappears on the edge of the field of view. At this point, the adjustment of the Kühler lighting system is completed. The micrographs taken are bright and clear, with normal contrast. Special attention should be paid to the following: a. The field diaphragm cannot be opened arbitrarily, but the field diaphragm can be reduced with the increase of the objective lens magnification. B. The height of the condenser should not be adjusted randomly, otherwise the adjusted Kühler illumination system will be damaged; When the objective lens is 10× or more, the front lens should be placed in the optical path; d. Regarding the matching of the objective lens multiple and the field diaphragm size, in the actual use process, it is not necessary to close the field of view or open the field of view as a general observation. However, for photomicrography, in order to avoid the interference of stray light on the photographic system and to take better pictures, the field diaphragm should be adjusted to just disappear when using the objective lens of each multiple. On the side of the observed field of view, this is a relatively complicated work, but it is necessary to do it. A simpler method is to adjust the field diaphragm corresponding to each multiple objective lens in advance, and make a mark for later use. Adjust directly to the corresponding position according to the mark.
