Determination of sugars in converted sugars by refractive rotation method
Carbohydrates are important excipients in pharmaceutical preparations. The Chinese Pharmacopoeia includes glucose as a nutritional drug and sucrose as a commonly used flavor correction agent in pharmaceutical preparations. Sucrose is a disaccharide formed by the polymerization of one molecule of glucose and one molecule of fructose. Its crystallinity allows it to exist at a high concentration in liquid or semi-solid formulations such as decoctions. During storage, it is prone to re crystallization, which affects the uniformity and stability of the formulation. In order to overcome crystallization, improve sweetness and antioxidant properties, and facilitate shaping, sucrose is dissolved in a small amount of water and held at 110-115 ℃ for 2 hours in the presence of 0.1% tartaric acid to produce invert sugar. Like honey, invert sugar is mainly chemically formed as a mixture of glucose and fructose. Many enterprises use it instead of sucrose and honey in the pharmaceutical and food industries. In order to control the quality of invert sugar and its formulations, we used the Repo-3 invert sugar refractometer to determine the content of glucose and fructose in invert sugar and decoction, which can evaluate the conversion rate of invert sugar during the conversion process and the final product stage.
The sucrose and honey in the ointment must be refined and added to remove impurities, kill microorganisms, reduce moisture, and prevent the phenomenon of "sand return" (crystallization of sugar after storage for a certain period of time) in the ointment. The method of refining sugar is to add half of the sugar content in water and 0.1% tartaric acid to sucrose, heat and dissolve it, keep it slightly boiling, until the sugar liquid turns golden yellow, and the conversion rate reaches 40% to 50%.
The sand return is related to the total sugar content and converted sugar in the decoction. The total sugar content should be controlled below 85%, and the conversion rate should be controlled between 40-50%. The Repo-3 sugar conversion optical rotation all-in-one machine can quickly measure the content of various sugars in decoctions, including fructose, glucose, and sucrose.
conclusion
Traditional quantitative methods often utilize the reducing properties and liquid chromatography properties of sugars, and use methods such as redox titration and chromatographic determination to quantify. When dealing with the proportion of sugar content in mixed states and drug formulations, it is prone to interference and reduces measurement accuracy. The Repo-3 conversion sugar refractive rotation all-in-one machine is more suitable for measuring the content and proportion of sugars in mixed states.
Calibration standard solution for ATAGO refractometer and sugar meter
The ATAGO refractometer and sugar meter are calibrated for all products before shipment, and there will be no deviation from the scale unless an accident occurs during shipment. But if deviation occurs after long-term use or external impact, it is necessary to correct the zero point. For refractometers that cannot be zeroed with water, please use a standard solution to correct the zero point.
