Exploring the Causes of Blurred Edges in Microscopic Imaging
The main optical components of microscope imaging, such as lenses, apertures, observation tubes, and lenses, are all circular designs. Optical distortion due to assembly and design can result in blurred image edges. If only the edge of the interface image taken by the microscope is blurred, it is mainly caused by the optical mismatch of the C-mount and the camera adapter and the microscope or a design defect.
How to obtain high-definition microscopic images from ordinary civilian cameras, and the acquisition of microscope images by ordinary digital cameras, special camera adapters are essential. A good camera adapter needs to have one feature: high transmittance, flat field compensation, low dispersion, high coaxiality of the inner lens, accurate image size, and accurate distance. When purchasing, consumers need to consider the following issues:
1. Is there flat field compensation?
2. Dispersion situation;
3. Imaging target surface;
4. Whether the distance is accurate.
The main reasons for black edges and blurred edges in the photomicrography process are as follows:
1. The microscope and the camera are not synchronized, and the fine-tuning knob can be adjusted to be clear;
2. The field diaphragm of the microscope is too small;
3. The aperture diaphragm of the microscope is too large;
4. The size of the target surface of the C interface and the digital camera does not match;
5. The field of view of the microscope is too small;
6. The microscope condenser is eccentric.
