How many kinds of laser Confocal microscopy?
The laser Confocal microscopy mainly consists of microscope, laser light source, scanning device, detector, computer system (including data acquisition, processing, conversion and application software), image output device, optical device and confocal system.
The light collected by a regular optical microscope is a combination of non measurement light from above and below the focal plane, as well as reflected and diffracted light from the sample, resulting in low resolution. The Confocal microscopy uses the confocal principle to filter the stray light formed by the non measuring light spot on the focal plane and the reflected light and diffracted light of different focal planes of the sample, so that only the light of the focal plane passes through the detection pinhole, greatly improving the image quality.
There are two main types of laser Confocal microscopy. One is that a laser scanning device is added on the basis of fluorescence microscope imaging, and the resolution of optical imaging is improved by 30% -40% by using computer for image processing. The fluorescence probe is excited by ultraviolet or visible light to obtain fluorescence images of the internal microstructure of cells or tissues. Physiological signals such as Ca2+, PH value, Membrane potential and changes in cell morphology are observed at the subcellular level, Becoming a new generation of powerful research tools in fields such as morphology, molecular biology, neuroscience, pharmacology, genetics, etc; The second is to use confocal technology as the principle, combined with precision Z-scan modules, 3D modeling algorithms, etc., to non-contact scan the device surface and establish surface 3D images, which are used as detection instruments for micro and nano level measurement of various precision devices and material surfaces.
