How many types of laser confocal microscopes are there?
The main components of laser confocal microscope include: microscope, laser light source, scanning device, detector, computer system (including data acquisition, processing, conversion, application software), image output device, optical device and confocal system.
Ordinary optical microscope collects light from the focal plane above and below the non-measured light and the sample reflected light, diffracted light superposition, low resolution. The confocal microscope uses the confocal principle to filter the stray light formed by the non-measured light points on the focal plane and the reflected and diffracted light from the different focal planes of the sample, so that only the light from the focal plane passes through the detection pinhole, which greatly improves the image quality.
There are two main types of laser confocal microscope, one is a fluorescence microscope imaging based on the addition of a laser scanning device, the use of computer image processing, the resolution of optical imaging increased by 30% -40%, the use of ultraviolet or visible light excitation of fluorescent probes, so as to obtain the fluorescence image of the microstructure of the cells or tissues inside the sub-cellular level to observe such as Ca2+, PH value, membrane potential, etc. Physiological signals and changes in cellular morphology, becoming a new generation of powerful research tools in the fields of morphology, molecular biology, neuroscience, pharmacology, genetics, etc.; Secondly, it is an inspection instrument for micro and nano level measurement of various precision devices and material surfaces, based on the principle of confocal technology, combining with the precision Z scanning module and 3D modelling algorithms, etc., which can scan the surface of the device in a non-contact manner and build up a 3D image of the surface. Inspection Instrument.
