How to adjust a microscope to make the image clearer
The application of microscopes in modern science has become widespread.
Microscopes are classified into optical microscopes and electron microscopes according to their major categories.
Optical microscopes can be divided into transmission and reflection types based on the form of the optical path;
Electron microscopes can be divided into transmission and electron scanning types. The difference from optical microscopes is that their resolution is greatly enhanced. However, they generally require samples to be placed in a vacuum chamber, and some samples are not suitable.
Here, a common reflective optical microscope is used as an example to illustrate the adjustment of imaging methods, and the principle of transmission is the same.
Optical microscopes mainly image through objective lens and eyepiece lens, which are usually equipped with different magnification lens groups. By combining, a very wide range of magnification can be formed. This configuration is because although the high magnification lens group displays clear details, the field of view and depth of field are narrow, making it difficult to move in different target areas. Although the low-power lens group has a small magnification, its field of view and depth of field are both large, making it easy to search for targets over a large area. Moreover, certain specific samples do not require a large magnification, but require all targets within the field of view to be as clear as possible, so low magnification lenses are also useful. By combining the two, perfect and clear imaging can be achieved.
Step method:
Turn on the light source
Rotate the coarse adjustment handwheel to pull the objective lens group away from the stage to a safe distance
Based on experience, replace with a low magnification objective lens and a suitable eyepiece lens
Place the samples that have undergone necessary processing on the loading platform
Adjust the pupil distance of the eyepiece
Move the sample on the stage, search for the sample and target area, and observe and select the target through rough adjustment of the handwheel
Replace the appropriate high-power objective lens and eyepiece, and carefully focus by adjusting the handwheel
After obtaining clear imaging, research can be carried out, and if necessary, photos can be taken
After completing the work, turn off the light source and remove the sample. The objective lens group and eyepiece lens group must also be removed and stored in a dedicated, dry, and cool place, such as a drying bottle.
2, Attention:
Focusing technique: When fine-tuning the focus, there must be a process of blurring, clarity, and then blurring again to confirm the most accurate focus point found. Therefore, it is necessary to slightly tilt the head and then rotate back to the clear focus position.
For certain specific shaped samples, color filters can be chosen to improve the clarity of details. For example, for substances sensitive to narrow wavelengths and samples that have been fluorescently stained, specific color filters can be inserted into the optical path. In addition, in order to observe the special tissue in metal samples, polarizing plates can be inserted, the angle can be adjusted, and the tissue condition can be studied by observing the specific polarized light reflected by them.
