How to adjust the microscope to make the sample image clearer
Microscopes have been widely used in modern science.
The types of microscopes are divided into optical microscopes and electron microscopes according to their large categories.
Optical microscopes can be divided into transmissive and reflective types according to their optical path forms;
Electron microscopes can be divided into transmissive and scanning electron microscopes. The difference between electron microscopes and optical microscopes is that the resolution is greatly enhanced. However, it is generally required that the sample be placed in a vacuum chamber, and some samples are not suitable.
Here, we take the common reflective optical microscope as an example to illustrate the adjustment of imaging methods, and the principle of transmission is the same.
Optical microscopes mainly image through objective and eyepiece groups, which are usually equipped with different magnification lens groups. By combining, a very large magnification range can be formed. This configuration is because although the details of the high-power lens group are displayed clearly, the field of view and depth of field are narrow, making it difficult to move in different target areas. Although the magnification of the low magnification lens group is small, the field of view and depth of field are both large, making it easy to search for targets over a large area. Moreover, certain specific samples do not require a high magnification, but all targets within the field of view are required to be as clear as possible, so low magnification lenses also have their place of use. The combination of the two can achieve perfect clear imaging.
Notes:
Focusing technique: When fine-tuning the focus, there must be a blurry clear re blurry process to confirm the most accurate focus point found, so it is necessary to slightly turn over and then return to the clear focusing position.
For certain samples with specific shapes, color filters can be selected to improve the clarity of details. For example, for substances sensitive to narrow wavelengths and samples that have undergone fluorescence staining, specific color filters can be inserted into the optical path. In addition, to observe the special structure in the metal sample, a polarizer can be inserted, the angle can be adjusted, and the tissue condition can be studied by observing the specific polarized light reflected by it.
