How to adjust the microscope to obtain a clearer image of the sample
Optical microscope can be divided into transmission type and reflection type according to the difference of optical path form;
Electron microscope can be divided into transmission and scanning electron microscope, and optical microscope is different from the separate rate greatly enhanced, but generally require samples placed in a vacuum chamber, some samples are not suitable.
Here to common reflective optical microscope as an example to illustrate the adjustment of the imaging method, the same principle of transmission type.
Optical microscope mainly through the objective lens group and eyepieces into the image, objective lens and eyepieces are usually equipped with different magnification of the mirror group. Through the combination can be formed across a very large magnification range, this configuration is because the high-magnification lens group although the details show clear, but the field of view and depth of field is narrow, not easy to move in different target areas. The low magnification group has a small magnification but a large field of view and depth of field, making it easy to search for targets over a wide area. In addition, some specific samples do not require a large magnification, but require that all targets in the field of view are as clear as possible, so the low magnification lens group is also useful. Both of them can be used to obtain perfectly clear images.
Switching on the light source
Turn the coarse adjustment handwheel and pull the objective group away from the stage to a safe distance.
Replace the low-power objective set with a suitable eyepiece set according to experience.
Place the sample on the stage after the necessary treatment.
Adjust the pupil distance of the eyepieces
Move the sample through the stage, search for the sample and the target area, observe and select the target with the coarse adjustment handwheel
Replace the objective and eyepieces with appropriate high magnification lenses and focus carefully with the fine adjustment handwheel.
Obtain a clear image for study and take photographs if necessary.
When finished, the light source is switched off and the sample is removed. The objective and eyepiece sets must usually be removed and stored in a special dry, cool place, such as a desiccator bottle.
