When choosing which system to use or buy, the first consideration is which parameters are most important to the experiment: speed, sensitivity, resolution, multi-wavelength discrimination, or cell viability, many of which The parameters are in conflict with each other and need to be compromised. The second consideration is whether the purpose of using this system is for some specific detection, or for general detection. Generally speaking, systems with greater flexibility in hardware selection are more suitable for specific inspections, but have higher technical requirements for assembly and use. Choosing a system with mature software saves time. Whatever type of system you choose, test it with your own experimental materials first. The company's sales representative must select the appropriate filter, excitation light source and objective lens according to the fluorescent dye used in the experiment and the required magnification when demonstrating the equipment. In addition to the biological material of interest, a series of slides containing different dyes should be used to compare different systems based on the quantitative data obtained.
microscope
The first thing to do is to choose between an upright (viewed from above) or an inverted (viewed from below) microscope. In general, inverted microscopes are advantageous when observing living cells and obtaining high-resolution 3D images. The stage of the inverted microscope makes it easier for experimenters to manipulate samples, facilitate microinjection, and facilitate the use of environmentally controlled incubators. At the same time, the inverted microscope also provides better mechanical stability for the installation of the CCD camera.
Objective lens selection
The choice of objective lens and its usage mode is an important parameter that affects image quality. Choosing the right microscope objective for the mounting medium is critical for imaging. Microscope objectives can function in air (usually low magnification), water immersion (WI) or glycerol solution (80%), silicone oil, or oil for conventional objectives (RI 1.50 to 1.534). The objective lens is the most important part in a microscope and must be inspected and cleaned regularly.
