Optical microscope objectives are all derived from the Gaussian formula 1/'- 1/' = 1/f. For a fixed focal length objective, once the system is determined, the focal length f remains unchanged, and the image distance / also remains unchanged. Therefore, only by adjusting the position of the object surface along the optical axis, a clear objective image (generally called the primary image of the objective) can be obtained. The primary image of the objective lens passes through the optical interface (essentially a projection objective lens), and the primary image of the objective lens is re-imaged on the CCD light target surface. It can be seen that the so-called focusing refers to the working process of changing the relative position of the object surface and the objective lens along the light axis direction, so that the relationship between the object image and the Gaussian relationship is satisfied, so as to obtain a clear initial image of the objective lens. As mentioned above, there are two types of focusing techniques: manual and automatic. Automatic focusing can be divided into direct focusing and indirect focusing according to the method of signal detection. Image processing method auto-focusing is a direct focusing method. This method realizes auto-focusing by image processing on the imaging quality, which is a result of the Gaussian formula 1/I'- 1/I' for microscope imaging than optical microscope objective lens. = 1/f'. For a fixed focal length objective, once the system is determined, the focal length f remains unchanged, and the image distance I also remains unchanged. Therefore, only by adjusting the position of the object surface along the optical axis, a clear objective image (generally called the primary image of the objective) can be obtained. The primary image of the objective lens passes through the optical interface (essentially a projection objective lens), and the primary image of the objective lens is re-imaged on the CCD light target surface. It can be seen that the so-called focusing refers to the working process of changing the relative position of the object surface and the objective lens along the direction of the optical axis, so that the relationship between the object image and the Gaussian relationship is satisfied, so as to obtain a clear initial image of the objective lens.
As mentioned above, there are two types of focusing techniques: manual and automatic. Automatic focusing can be divided into direct focusing and indirect focusing according to the method of signal detection. Image processing method auto-focusing is a direct focusing method, which realizes auto-focusing through image processing of image quality, which is an ideal auto-focusing method for microscopes. The developed automatic microscope focusing device also realizes automatic focusing based on image processing method.
