How to Improve Microscope Resolution
1. Improve the mirror port rate;
Wavelength 0.55; N.A1.4, then R=0.55/2×1.4=0.2 microns
The resolution limit of ordinary microscopes is 0.2 microns
2. Shorten the wavelength of ultraviolet light, then R=0.27/2×1.4=0.1 microns
can double the resolution (fluorescence microscope)
Using shorter X and γ-rays, the wavelength of the electron flow in the electron microscope is 0.0387A
2. Change the injection mode of the microscope, from direct to oblique, and reflect the light microscope in the dark, double the resolution, and observe the living body.
3. Change the structure of the short wave, increase the peak; reduce the trough, use 1/2 peak or trough, so as to improve the resolution, such as phase contrast microscope. In 1952, he won the Nobel Prize. Resolution 0.1 micron (living body, positive and negative phase difference)
4. Use 1/4 wavelength to improve resolution, such as differential interference microscope
