How to use a fluorescence microscope
1. Turn on the light source, and the ultra-high pressure mercury lamp needs to warm up for a few minutes to reach the brightest point.
2. The transmission fluorescence microscope needs to install the required excitation filter between the light source and the condenser, and install the corresponding blocking filter behind the objective lens. Epi-fluorescence microscopes need to insert the required excitation filter/dual-color beam splitter/blocking filter inserts into the slots in the light path.
3. Observe with a low-magnification lens, and adjust the center of the light source so that it is located in the center of the entire illumination spot according to the adjustment device of different models of fluorescence microscopes.
4. Place the specimen piece and observe after focusing. Attention should be paid during use: do not observe directly with the end filter, so as not to cause eye damage; when observing specimens with an oil lens, you must use a special oil lens without fluorescence; after the high-pressure mercury lamp is turned off, it cannot be turned on again immediately, it needs to be tested. It can be restarted after 5 minutes, otherwise it will be unstable and affect the life of the mercury lamp.
