How to use oil lens of 100X microscope
Oil lenses are inevitably used when microscopes use high magnification objectives. Especially the 100X objective lens, the 100X objective lens is divided into two types: dry lens and oil lens. As the name suggests, dry lens does not need to use oil, while oil lens needs to use oil.
Microscopes are divided into two types: upright and inverted. Based on the microscope workbench, the objective lens of the upright microscope is above the workbench, while the inverted one is below the workbench.
The oil lens of the upright microscope is to drop the lens oil on the surface of the object, slowly adjust the coarse adjustment and fine adjustment, pay attention to the descending distance of the objective lens, and align the objective lens with the lens oil dripped on the surface of the object, and adjust the oil lens to the surface of the object. When the lens is immersed in oil droplets, you can observe it from the eyepiece and adjust the coarse and fine adjustments to make the lens focus.
Since the objective lens of the inverted microscope is under the worktable, the surface of the object to be observed is downward. If we drop the mirror oil on the surface of the object and turn the object to observe, the mirror oil will drip, so we need to drop the mirror oil on the surface of the object. on the microscope objective.
At this time, we can use the worktable as the benchmark, adjust the objective lens to the height, drop the lens oil on the objective lens, the lens oil will protrude into droplets, and then move the objective lens down slowly, so that the objective lens is slightly lower than the stage , the purpose is to prevent the object from touching the objective lens, so as not to damage the objective lens, and then place the object on the stage, adjust the focal length, and observe.
After using the oil lens, please clean the objective lens with a mixture of absolute alcohol and yi ether. Wipe with lens tissue or absorbent cotton (filament cotton). The ratio of absolute alcohol and yi ether is 2:8. Do not wipe in heavy dust or very dry environments.
