Introduction to the principles of fluorescence microscopy
1, fluorescence microscope is to use ultraviolet light as a light source, used to irradiate the object to be examined, so that it emits fluorescence, and then observe the shape of the object and its location under the microscope. Fluorescence microscope is used to study the uptake and transport of substances in the cell, the distribution and localisation of chemicals, etc. Some substances in the cell, such as chlorophyll, can be found in the cell. Some substances in the cell, such as chlorophyll, can fluoresce after irradiation by ultraviolet light; there are some substances that cannot fluoresce by themselves, but if stained with fluorescent dyes or fluorescent antibodies, they can also fluoresce after irradiation by ultraviolet light, and fluorescence microscope is one of the tools to carry out qualitative and quantitative research of such substances.
2,Principle of fluorescence microscope:
(A) light source: the light source radiates various wavelengths of light (in the ultraviolet to infrared).
(B) excitation filter source: through the specimen can produce fluorescence of a specific wavelength of light, while blocking the excitation of fluorescence useless light.
(C) Fluorescent specimen: generally stained with a fluorochrome.
(D) Blocking filters: block out excitation light that is not absorbed by the specimen to selectively transmit fluorescence, and some wavelengths in the fluorescence are also selectively transmitted.
A microscope that uses ultraviolet light as a light source to make the irradiated object fluoresce. The electron microscope was first assembled in 1931 in Berlin, Germany by Knorr and Haroska. This microscope uses a high-speed electron beam instead of a light beam. Because the wavelength of the electron stream is much shorter than the light wave, so the magnification of the electron microscope can reach 800,000 times, the minimum limit of resolution of 0.2 nm. 1963 began to use the scanning electron microscope can make people see the surface of the object's tiny structure.
3, the scope of application: used to magnify the image of tiny objects. Generally used in biology, medicine, microscopic particles and other observations.
