Magnification calibration and solutions when using microscopes for measurement
The magnification calibration of microscope measurement refers to debugging and calibrating the entire set of products before measuring the object to be measured.
Commonly used equipment is: microscope (biological, stereoscopic or metallographic), measurement software, light source and objective lens ruler (complete equipment and objective lens ruler).
The specific steps are as follows: Adjust the microscope to the required magnification, place the objective lens ruler under the microscope in the X direction, then adjust the focal length until the observed objective lens ruler is clearest, open the measurement software, enter the calibration menu, first Calibrate in the X direction and align the calibration line of the software with the measurement lines on the objective lens scale. (Screenshot of measurement calibration) The more lines there are, the smaller the error. After confirmation, a dialog box will appear. Enter the actual size in the dialog box ( Measurement calibration screenshot), for example, the actual size of a line is 0.01mm, and the number of lines we align is 10, then enter 0.1mm in the dialog box so that our X direction is calibrated, and then set the objective lens ruler Reverse the direction, adjust to the Y direction, and repeat the above steps until the Y direction calibration is completed.
Notes: 1. Different software versions have different calibration methods. Please refer to the operation manual before use. The operation manual shall prevail. 2. Different multiples require calibration. Do not use one calibration coefficient on multiple multiples to avoid affecting the measurement accuracy. Try to calibrate all the multiples that can be used, and then store them in the system. When using different multiples, call up the coefficients corresponding to the multiples.
