Matters needing attention when using a microscope with a digital camera
The use of digital cameras for microscopy photography has become a very effective means today. Convenient and fast imaging; on-the-spot preview, save, delete or print make photomicrography a new development.
What use digital camera to shoot microscopic image at present is mostly digital digital camera (as NIKON4500, CANONG6, A620 etc.), the camera that both camera lens is not detachable. In this way, an intermediate lens needs to be added between the microscope and the camera lens, so that the microscopic image is formed on the focal plane of the camera --- we call it a relay lens.
The quality of the lens directly affects the quality of the captured microscopic image. Given that there is no uniform inspection standard for relay mirrors at present, and there is no consensus between users and producers, it is difficult to distinguish good from bad. Therefore, there are many manufacturers, and the relay mirrors on the market are mixed with good and bad. According to our many years of working on microscopes and our understanding of digital photography, and comparing the pictures taken by various relay mirrors at home and abroad, we have summarized some methods to identify the pros and cons of relay mirrors for your reference:
one. image size
It is generally believed that adding a relay lens as long as the image can fill the camera
The LCD screen is a success, and it is a qualified relay mirror. It is only a minimum standard for the relay mirror to fill the screen, and some cameras have to use up the optical zoom of the camera to fill the screen. The optical system of such a relay mirror is defective.
two. Restoration of image color
The reducibility of the color of the microscopic image is also a standard for testing the relay mirror. A qualified relay mirror requires that the color of the image taken out is consistent with the color of the image observed by the microscope.
three. image depth of field
Anyone who knows how to take pictures knows that shooting an image requires a certain depth of field (art photography is an exception). When we observe microscopic images, we all have a certain sense of hierarchy. A qualified relay mirror must have a depth of field no less than that of the microscope objective.
Four. image sharpness
This is the most important indicator for testing relay mirrors. The so-called "sharpness" is what we usually call resolution, and the level of this index directly affects the quality of the captured microscopic image.
In short, the ultimate criterion for identifying a relay mirror is to ensure that the quality of the image captured by the digital camera and the image observed in the microscope are consistent to the greatest extent.
