Methodological steps in the use of a microscope
1. Place the microscope on the table during the experiment, the mirror base should be about 6-7cm from the edge of the table, and turn on the bottom light source switch.
2. Turn the converter so that the low-magnification lens is facing the light-through hole on the carrier table. Then gaze into the eyepiece with both eyes, adjust the intensity of the light source, raise the spotting scope, adjust the iris aperture to the maximum, so that the light is reflected into the mirror briefly, which is bright in the field of view.
3. Place the mount to be observed on the carrier table so that the part to be observed is located in the centre of the light-through hole.
4. Observe first with low magnification (objective lens 10×, eyepiece 10×). Before observation, turn the coarse focusing screw to raise the stage so that the objective lens gradually approaches the section. Care should be taken so that the objective lens does not touch the slide to prevent the lens from crushing the slide. Turn the coarse focusing screw to slowly lower the stage, and you will soon see a magnified image of the material on the slide.
5. If the object image seen in the field of view does not meet the requirements of the experiment (the object image deviates from the field of view), you can slowly move the left and right moving ruler. When moving the slide should be noted that the direction of movement and the field of view to see the image of the object moving in the opposite direction. If the object image is not very clear, you can adjust the fine focusing spiral, until the object image is clear.
6. If you further use the high-magnification objective observation, should be converted before the high-magnification objective, the object image needs to be enlarged to observe the part of the field of view to the centre (will be converted from low-magnification objective to high-magnification objective observation, the field of view of the object in the scope of the narrowing of a lot). General with the normal function of the microscope, low-power objective lens and high power objective lens is basically in focus, in the low-power objective lens observation is clear, change the high-confidence objective lens should be able to see the object image, but the object image is not necessarily very clear, you can turn the fine focusing screw to adjust.
7. In the conversion of high-magnification objective and see the object image, according to the need to adjust the aperture or concentrator, so that the light meets the requirements of the general will be changed from low-magnification objective to high-magnification objective observation, the field of view to be a little darker, so you need to adjust the intensity of the light).
8. Observation is completed, the objective lens should be removed from the light hole, and then the microscope recovery. And check the parts have no damage (special attention should be paid to check whether the objective lens is water, such as stained with water to be wiped with lens paper), check the processing can be put back to the original place after the completion of.
