Multiply of red objective lens of optical microscope
The red objective of the optical microscope indicates that the magnification is 4 times, yellow represents 10 times, blue represents 40 times and white represents 100 times. Multiply the eyepiece multiple by the objective multiple to get the total magnification of the microscope.
The "10" and "4" in the number on the optical microscope indicate that the magnification of the objective lens is 10 times or 4 times, where the magnification is not the magnification of the area, but the magnification of the length or width of the image, and the total magnification of the microscope is equal to the product of the magnification of the objective lens and the eyepiece, and the magnification of the objective lens is directly proportional to its length. The greater the magnification of the objective lens, the longer the objective lens.
Parameter relation of optical microscope
The working distance of the objective lens of an optical microscope is related to the focal length of the objective lens. The longer the focal length of the objective lens, the lower the magnification and the longer the working distance. Example: The 10x objective lens is marked with 10/0.25 and 160/0.17, where 10 is the magnification of the objective lens. 0.25 is the numerical aperture; 160 is the lens barrel length (mm); 0.17 is the standard thickness of cover glass (mm).
The effective working distance of 10x objective lens is 6.5mm, and that of 40x objective lens is 0.48mm. The objective lens is used to enlarge the specimen for the first time, and it is the most important component resolution that determines the performance of the microscope.
When the fine structure has been clearly distinguished by the objective lens, if it is not magnified by the eyepiece and cannot reach the size that can be distinguished by the human eye, it will not be clear; However, the fine structure that can't be resolved by the objective lens can't be seen clearly, although it is magnified by the high-power eyepiece, so the eyepiece can only play a magnifying role and will not improve the resolution of the microscope.
