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Notes on the use of optical microscopes in biology

Jan 04, 2024

Notes on the use of optical microscopes in biology

 

1. Hold the microscope with the right hand holding the arm and the left hand resting on the seat position, not one-handed extraction, so as to avoid parts falling off or collision to other places.


2. Hold and place the microscope lightly, do not place the microscope on the edge of the experimental bench, so as not to knock over to the ground.


3. Keep the microscope clean, optical and lighting parts can only be wiped with mirror paper, do not blow hand wipe or wipe with a cloth, mechanical parts with a cloth.


4. Water droplets, alcohol or other drugs do not touch the lens and mirror stage, if stained should be wiped clean immediately.


5. Place the slide specimen should be aligned with the centre of the fluorescent hole, and can not put the slide in reverse, to prevent pressure damage to the slide or touch the objective lens.


6. To develop the habit of keeping both eyes open at the same time, the left eye to observe the field of view, the right eye for drawing.


7. Do not remove the eyepiece to prevent dust into the objective lens, and do not arbitrarily disassemble the various parts to prevent damage.


8. After use, must be restored to be put back into the mirror box, the steps are: remove the specimen piece, rotate the rotator so that the lens away from the light hole, down the mirror stage, flat reflector, down the light collector (but do not touch the reflector), close the aperture, the push piece of equipment back to cover the silk cloth and the outer cover, back to the lab bench cabinet. Finally, fill in the use registration form. (Note: the reflector should usually be placed vertically, but sometimes because the light collector is not raised to the height it should be, the aperture will be touched when the mirror table is lowered, so it is changed to be placed horizontally).

 

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