Operating procedures and optical microscope upkeep techniques
1. Place
Hold the mirror arm with your right hand and support the mirror base with your left hand to keep the mirror body upright. The desktop should be clean and stable, and you should choose a place near a window or with sufficient light. The single tube is generally placed on the left side, 3 to 4 cm away from the edge of the table.
2. Clean
Check whether the microscope is faulty and clean, and the mechanical parts of the mirror body can be wiped with a clean soft cloth. The lens should be wiped with lens cleaning paper. If there is glue or dirt, clean it with a small amount of xylene.
3. To the light
The lens barrel is raised to a distance of 1-2 cm from the stage, and the low power lens is aligned with the light hole. Adjust aperture and reflector, use flat mirror when light is strong, use concave mirror when light is weak, reflector will turn with both hands.
If a microscope with a light source is used, this step can be omitted, but a knob for adjusting the brightness is required.
4. Install the specimen
Place the slide on the stage with the cover glass facing up. Secure the slide with spring clips, and turn the knob of the stage mover so that the material to be observed is aligned with the center of the aperture.
5. Focus
When adjusting the focus, first turn the coarse focus knob to slowly lower the lens barrel, and observe carefully from the side until the objective lens is close to the slide specimen, then observe from the eyepiece with the left eye, and turn the coarse focus knob with the left hand to raise the lens barrel until the target lens is clearly visible. Stop when the object is imaged, and then use the fine focus knob to adjust it back clearly.
Operation Note: Do not adjust the focus directly under a high-magnification lens; when the lens barrel is lowered, observe the distance between the lens barrel and the specimen from the side; know the critical value of the object distance.
If you use a binocular microscope, if there is a difference in the diopter of the observer's eyes, you can use the diopter adjustment circle to adjust it. In addition, the binoculars can be relatively translated to adapt to the distance between the operator's eyes.
