Optical principles and accessories for light microscopesOptical principles and accessories for light microscopes
Principle of Optics
Microscope is the use of convex lens magnification imaging principle, the human eye can not distinguish the tiny objects enlarged to the size of the human eye can distinguish, the main thing is to increase the near tiny objects to the eye angle (angle of view of the large object in the retina image is large), with the angular magnification of the M said that the magnification of the ability of their. Because of the same object to the eye angle and the distance of the object from the eye, so the general provisions of the image of the distance from the eye for 25 cm (visual distance) at the magnification of the instrument for the magnification. The angle of view of an object observed by a microscope is usually very small, so the ratio of the angle of view can be replaced by the ratio of its tangent.
A microscope consists of two converging lenses, and the optical path is shown in the diagram. Object AB through the objective lens into a magnified inverted solid image A1B1, A1B1 is located in the eyepiece of the focal length of the object side of the inner, through the eyepiece into a magnified virtual image of A2B2 in the distance of the bright vision.
Accessories
With the development of modern biotechnology and the improvement of people's requirements for microscopes, a single optical micro-imaging system has been far from being able to meet the requirements of people's micro-photography. The introduction of digital microscope marks the entry of optical microscope into a new digital era. Digital microscope not only combines the good imaging characteristics of optical microscope, but also combines it with advanced photoelectric conversion technology and liquid crystal screen technology, so that the microscope has the ability to observe microscopically, and at the same time, it realises the digital storage and transmission of microscopic images.
However, the high cost of digital microscope does not make it widely used, a new type of microscopic digital products - microscopic digital camera also came into being. As a special digital microscope camera, the digital microscope camera can be easily linked to any microscope to achieve the conversion of optical microscope to digital microscope.
As a necessary accessory for optical microscopes, micro-digital cameras are also divided into many different grades according to different needs, some are more suitable for higher demands on images, and some are more suitable for generalised needs. As a convenient and fast microscope camera system, the microscope digital camera will be greatly used.
Optical microscope accessories are eyepiece objective lens and light source, etc. Of course, with the development of science and technology, optical microscope gradually also equipped with a camera system, so that it can be displayed on the monitor, viewing time is also more convenient.
In addition, the light source of the light microscope is also more important, equipped with a ring light source when there is also a light source, clip type light source. Optical microscope base is not the same, some are made of plastic, some are alloy, the better is the alloy material, alloy is not easy to deform. The magnification is also not the same, some can be continuously doubled, some only a multiplier, or two times.
