Precautions for using a microscope in conjunction with a digital camera
Digital cameras have become a very effective means for microscopic photography today. Convenient and fast imaging; Previewing, saving, deleting, or printing on the spot has led to new developments in micrography.
At present, most of the digital cameras used for capturing microscopic images are digital cameras (such as NIKON4500, CANONG6, A620, etc.), which are non detachable cameras with camera lenses. This requires adding an intermediate lens between the microscope and the camera lens to make the microscopic image appear on the focal plane of the camera - we call it a relay lens.
The quality of this lens directly affects the quality of the captured microscopic image. Given that there is currently no unified inspection standard for relay mirrors, and there is no consensus between users and producers, it is difficult to distinguish between good and bad. Therefore, many manufacturers have emerged, and the relay mirrors in the market are mixed with good and bad. Based on our years of work in microscopes and our understanding of digital photography, we have compared various pictures taken by relay mirrors at home and abroad, and summarized several methods for identifying the advantages and disadvantages of relay mirrors for your reference:
1、 Image size
It is generally believed that with a relay mirror, as long as the image can fill the camera
The LCD screen is a success, it is a qualified relay mirror. The ability of a relay mirror to fill the screen is only a minimum standard, while some require the camera's optical zoom to be used up to make the screen full. This type of relay mirror's optical system is flawed.
2、 Restoration of Image Colors
The color restoration of microscopic images is also a standard for testing relay mirrors. A qualified relay mirror requires the color of the image taken to be consistent with that observed by the microscope.
3、 The depth of field of an image
People who know photography know that shooting an image requires a certain depth of field (except for art photography). We all have a certain sense of hierarchy when observing microscopic images. A qualified relay lens should have a depth of field not less than the microscope objective.
4、 Sharpness of images
This is an important indicator for testing relay mirrors. The so-called "sharpness" refers to the resolution, which directly affects the quality of the captured microscopic images.
In short, the ultimate standard for identifying a relay mirror is to ensure that the quality of the image captured by a digital camera is consistent with that observed in a microscope to the greatest extent possible.
