Precautions for using fluorescence microscope
(1) Operate in strict accordance with the requirements of the fluorescence microscope factory manual, do not change the procedure arbitrarily.
(2) The inspection should be carried out in the dark room. After entering the darkroom, connect the power supply, ignite the ultrahigh-pressure mercury lamp for 5-15min, wait until the light source emits a strong light to stabilise, and the eyes are completely adapted to the darkroom, and then start observing the specimen.
(3) To prevent damage to the eyes from ultraviolet light, protective glasses should be worn when adjusting the light source.
(4) check the time each time to 1 ~ 2h is appropriate, more than 90min, ultra-high-pressure mercury lamp luminous intensity gradually decreased, fluorescence weakened; specimens irradiated by ultraviolet light 3 ~ 5min, fluorescence is also significantly weakened; therefore, * shall not be more than 2 ~ 3h.
(5) fluorescence microscope light source life is limited, the specimen should be centralised inspection, to save time and protect the light source. When it is hot, should be added fan cooling, new bulb should be recorded from the beginning of the use of time. When the lamp is extinguished and wants to be used again, the lamp must be cooled down sufficiently before it is lit. The light source should be avoided several times in **.
(6) Observe the specimen immediately after staining, as the fluorescence will gradually diminish over time. If the specimen is placed in a polyethylene plastic bag stored at 4 ℃, it can delay the time of fluorescence weakening and prevent the evaporation of the sealing agent.
(7) Judgement criteria for fluorescence brightness: generally divided into four levels, that is, 'one' - no or visible weak fluorescence. '+' - only clear visible fluorescence. '++' - visible bright fluorescence. '++++'-Bright fluorescence is visible.
