Procedure for using a microscope

Dec 05, 2023

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Procedure for using a microscope

 

Taking the mirror and placing it
1. Hold the mirror arm with your right hand and support the mirror base with your left hand.


2. Place the microscope on the experimental table, slightly to the left (the microscope is placed about 7 cm away from the edge of the experimental table)
place). Install the eyepiece and objective lens.


to light
3. Rotate the converter to align the low-magnification objective lens with the clear aperture (the front end of the objective lens should be kept 2 cm away from the stage).


4. Aim a larger aperture at the clear aperture. Keep your left eye looking into the eyepiece (open your right eye to facilitate drawing at the same time later). Rotate the reflector so that the light is reflected into the lens barrel through the light hole. Through the eyepiece, you can see a bright white field of view.


observe
5. Place the slide specimen to be observed (it can also be made from a thin piece of paper with a "6" printed on it) on the stage and press it with a press clamp. The specimen should be facing the center of the light hole.


6. Turn the coarse focus screw to slowly lower the lens barrel until the objective lens is close to the glass specimen (looking at the objective lens,
To prevent the objective lens from touching the slide specimen).


7. Look into the eyepiece with your left eye, and at the same time turn the coarse focus screw in the opposite direction to slowly raise the lens barrel until the object image is clearly visible.
end. Then turn the fine focus screw slightly to make the object image you see clearer.


8. Use of high-magnification objective lens: Before using high-magnification objective lens, you must first use low-magnification objective lens to find the object to be observed, and adjust it to
center of the field of view, then turn the converter and change to a higher magnification lens. After switching to a high-magnification lens, the brightness in the field of view becomes darker, so it is generally
Choose a wider aperture and use the concave side of the mirror, then adjust the fine focus screw. The number of objects viewed becomes smaller, but
Increased size.


tidy
8. After the experiment, wipe the surface of the microscope clean. Turn the converter, tilt the two objective lenses to both sides, and align the lenses
The barrel is slowly lowered to the lowest position and the reflector is placed vertically. Finally, put the microscope into the mirror box and return it to its original place.


"Precautions":
1. It is strictly forbidden to pick up the microscope with one hand.


2. If you need to move the microscope, be sure to lift the microscope and put it in the appropriate position. Do not push the microscope (it will cause damage when pushing).
Sustained vibration may cause the internal parts of the microscope to loosen, remember!!), please be careful when using the microscope.


3. When using a microscope, the height of the seat should be appropriate. When observing, you should be used to observing with both eyes at the same time, and the aperture and light source should be bright.
The temperature should be appropriate, otherwise it is easy to feel tired when observing for a long time.


4. When turning the rotating disk, be sure to lower the stage to the lowest point to avoid scratching the eyepiece lens due to improper operation.


5. The slide should be removed for specimen staining or any other operation. After the operation is completed, put it back on the stage for observation. Do not
Operate on the stage to prevent dye or other liquids from flowing into the inside of the microscope or damaging the lens.


6. After observing one material and want to change to another material, be sure to lower the stage to the lowest point. After changing the glass slide,
Refocus according to standard procedures. Do not replace specimens directly to avoid scratching the lens or specimen slides.


7. After using the microscope, lower the stage to the lowest point, align the low-magnification lens with the round hole in the center of the stage, roll up the power cord, cover it with a dust cover, and store it in the storage cabinet.

 

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