How should a spectrophotometer be zeroed? Specific steps are as follows:
1. Before using the UV spectrophotometer, the user should first understand the structure and working principle of the instrument, as well as the function of each operating knob. Before the power is turned on, the instrument should be checked, the power cord should be firmly wired, the ground should be good, the starting position of each adjustment knob should be correct, and then the power switch should be turned on.
2. Turn on the power, the indicator light is on, the selector switch is set to "T", and the wavelength is adjusted to the test wavelength. The instrument warms up for 30 minutes.
3. Open the cover of the sample chamber, adjust the "0" knob to make the number display "0. 00", cover the sample chamber cover, put the cuvette holder in the distilled water calibration position, make the photocell receive light, and adjust the transmittance" 100%" knob to make the word display "100.0"
4. After preheating, press (3) to adjust "0" and "100%" several times in succession, and the UV spectrophotometer can start the measurement.
5. Absorbance measurement: Press (3) to adjust "00.0" and "100%" of the instrument, set the selector switch to "A", adjust the absorbance zero adjustment knob to make the number display "000", and then set the measured The sample is moved into the optical path, and the displayed value is the absorbance value of the tested sample.
