Proper Maintenance of Focusing Laboratory Biological Microscopes

Apr 06, 2026

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Proper Maintenance of Focusing Laboratory Biological Microscopes

 

1. The placement of biological microscopes should be in a dry and clean room to avoid mold growth on optical components, rust on metal parts, and dust accumulation. After using the microscope, put it back into the box (cabinet), or cover it with a glass or plastic cover, and put in the drying agent.

 

2. Do not disassemble any components by yourself; The lens barrel should be inserted with an eyepiece or a shackle cover to prevent dust from entering from the upper part of the barrel; Do not touch or wipe the surface of the lens with your fingers. If there is dust, gently brush it off with a soft brush first, then use a soft clean cloth to wipe it off. You can also use lens paper dipped in a little xylene or petroleum jelly to try wiping, but be careful not to scratch stripes on the surface of the lens. If there is mild mold growth on the lens that cannot be wiped off with wiping paper, a cotton swab dipped in a mixture of 70% ethanol and 30% ethylbenzene can be used to gently wipe and rub.

 

3. Biological microscopes should not be placed together with corrosive acids, downgraded or highly volatile chemicals to prevent corrosion and shorten their service life. In principle, when observing specimens containing liquids, it is generally necessary to cover them with a glass slide; If the liquid contains corrosive chemicals such as acid or alkali, the surrounding area of the cover glass should be sealed with paraffin or Vaseline, and then observed. However, due to the frequent use of this type of reagent in the microscopic identification of traditional Chinese medicine, it is impossible to seal it all. Therefore, special care should be taken to prevent the liquid from flowing onto the stage and to avoid touching the objective lens.

 

4. Biological microscopes should not be exposed to direct sunlight, nor should they be placed near stoves or heaters to avoid excessive changes in temperature that can cause lens and mechanical components to detach, deform, or be damaged.

 

5. Cleaning the lens is limited to the outer surface. After the receiving surface is contaminated with drugs, use a mirror paper dipped in a small amount of lens cleaning solution to wipe it (do not use ethanol); If the back needs to be cleaned, a soft brush can be used to wipe it off, or a leather suction head can be used to remove dust.

 

6. When adjusting the thickness of the aluminum focus, the action should be slow and do not crush the cover to prevent damage to the objective lens and collector due to controlled impact.

 

7. After using the oil lens, the cedarwood oil on the lens must be wiped clean (a small amount of xylene can be dipped in lens cleaning paper to wipe, but xylene should not penetrate into the inside of the lens, otherwise xylene will dissolve the adhesive between the lenses and cause the lenses to fall off).

 

8. The mirror surface of the reflector should be protected and cleaned, and water, xylene, or cedarwood oil should not penetrate to prevent mercury from falling off the reflector.

 

9. If the mechanical part is not flexible, a fine silk cloth dipped in xylene can be used to wipe away rust and grease (ethanol should not be used as these solvents can corrode the paint), and then a small amount of liquid stone can be used for lubrication; Do not twist too tightly to avoid damage.

 

10. Sometimes stains or foreign objects are found in the field of view of a biological microscope. You can first rotate the eyepiece, and if these stains follow the rotation, it can be determined that the stains are on the eyepiece; Otherwise, the specimen can be moved, and if the stain moves with it, the stain is on the specimen. If neither is true, then the stain is on the objective lens. You can first pick up the front lens of the food mirror and then check the rear lens. Clean according to the situation.

 

Sometimes a part of the field of view is not clear, which may be due to fingerprints or dust on the surface of the front lens of the objective or eyepiece, or it may be due to poor preparation of the specimen or improper use of the microscope, such as the lighting system not being properly adjusted. The situation should be investigated and resolved separately.

 

11. After using the biological microscope, all parts should be wiped clean, the lattice objective lens should be transformed into a figure eight shape, then the tube and collector should be lowered and fixed, and the mirror surface of the reflector should be placed in a vertical position.

 

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