Steps in the use and maintenance of optical microscopes
Operation steps and precautions
(I) Orthostatic microscope
1. Hold the arm of the microscope with your right hand and hold the base with your left hand to keep the microscope body upright. The desktop should be clean and smooth, and you should choose a place near the window or with sufficient light. The monocular is usually placed on the left side, 3 to 4 cm from the edge of the table.
2, clean and check whether the microscope is faulty, whether clean, the mechanical part of the mirror body can be wiped with a clean soft cloth. The lens should be wiped with mirror paper, if there is glue or sticky dirt, a small amount of xylene can be used to clean it.
3, the light-aligning lens barrel to 1 ~ 2 cm from the carrier platform, low-magnification mirror aligned with the through-hole. Adjust the aperture and reflector, when the light is strong, use a plane mirror, when the light is weak, use a concave mirror, the reflector should be rotated with both hands. If you use a microscope with a light source, you can omit the sub-steps, but you need to adjust the brightness knob.
4,Mount the specimen by placing the slide on the carrier table, and note that the side with the coverslip must be facing up. Use the spring clip to fix the slide, turn the knob of the platform mover, so that the material to be observed is aligned with the centre of the light-through hole.
5, focus focus, first rotate the coarse focus knob slowly lower the barrel, and carefully observe from the side, until the objective lens close to the slide specimen, and then the left eye from the eyepiece observation, the left hand rotate the coarse focus knob to lift the barrel, until the specimen to see the image of the object to stop, and then fine focus knob back to adjust clear. Operation Note: should not be in the high power lens directly focus; barrel down, should be observed from the side of the barrel and the distance between the specimen; to understand the critical value of the object distance. If the use of binocular microscope, such as observers with binocular vision differences, reliable vision adjustment ring adjustment. In addition, the binoculars can be relative translation to adapt to the operator's eyes spacing.
6, observation, if using a monocular microscope, both eyes open naturally, the left eye to observe the specimen, the right eye observation records and drawings, while the left hand to adjust the focus, so that the object is clear and move the specimen field of view. Record and draw with the right hand. During microscopic examination, the specimen should be moved in a certain direction until the whole specimen has been observed, so that there is no omission and no repetition. Adjustment of light intensity: In general, the light of stained specimens should be strong, and the light of colourless or unstained specimens should be weak; the light of low magnification observation should be weak, and the light of high magnification observation should be strong. In addition to adjusting the reflector or light source lamp, iridescent aperture adjustment is also very important.
(1) low-magnification observation observation of any specimen, it is necessary to use low-magnification first, because of its large field of view, easy to find the target and determine the site to be observed.
(2) High magnification observation from low magnification to high magnification, only slightly mobilise the fine focusing knob, you can make the object image clear. Do not use the coarse focusing knob when using the high magnification lens, otherwise it is easy to crush the coverslip and damage the lens. When turning the objective converter, do not use your fingers to push the objective lens directly, which is easy to make the optical axis of the objective lens deflection, converter threads uneven force and damage, * and then lead to the converter will be scrapped.
(3) the observation of the oil mirror first with low magnification and high magnification mirror will be examined after the object moved to the centre of the field of view, and then change the oil mirror observation. Before oil mirror observation, the brightness of the microscope should be adjusted to * bright, aperture fully open. When using the oil mirror, first add a drop of cedar oil (mirror oil) on the coverslip, and then lower the barrel and carefully observe from the side until the oil mirror immersed in cedar oil and close to the slide specimen, and then observe with the eyepiece, and fine focus knob to raise the barrel, until you can see the specimen's focal length when the focus stops and adjusted clearly. Add the right amount of cedar oil. After the use of the oil mirror must be used to wipe off the cedar oil with xylene on mirror paper, and then wipe off the excess xylene with dry mirror paper.
7, the end of the operation observation is complete, remove the sample, twist the converter, so that the lens V-shaped bias on both sides, the reflector should be upright, lower the lens barrel, wipe clean, and put on the lens cover. If you are using a microscope with a light source, you need to adjust the brightness knob to adjust the light brightness to * dark, and then turn off the power button, in order to prevent the next time you switch on the instantaneous overcurrent burnt light source lamp.
