Steps to replace low magnification microscope with high magnification microscope
(1) Mirror retrieval and placement
Microscope is a high-precision optical experimental instrument. When not in use, it is usually packaged and placed in a box. When in use, it is taken out and opened. When taking out the mirror, hold the mirror arm tightly with your right hand and hold the mirror holder with your left hand to keep the mirror body upright to prevent the reflector and eyepiece from slipping and throwing out. Gently place the microscope on the experimental table, usually on the left side, 3-4 centimeters away from the edge of the table, with the mirror arm facing your chest for easy observation.
(2) Cleaning
Check if the microscope is faulty and clean. If there is dust or dirt on the metal parts, they can be wiped with a clean soft cloth. If there is dirt on the lens, it should be wiped with lens cleaning paper and never with a handkerchief. If there is glue or dirt, a small amount of xylene can be used to clean it.
(3) Light Steps
Raise the lens barrel to a distance of 1-2 centimeters from the stage, rotate the objective lens converter to turn the low-power mirror towards the stage, and align it with the central through-hole. During the rotation process, it should be slow. When you hear a clicking sound, open the aperture, raise the light collector, and turn the reflector towards the light source. Observe with the left eye on the eyepiece (do not close the right eye tightly), while adjusting the direction of the reflector until the light in the field of view is uniform and bright.
(4) Place glass slide specimens
Take a glass slide specimen and place it on the stage, making sure that the side with the cover glass is facing upwards, otherwise it will not be able to focus when observed with a high-power microscope, and the glass slide specimen is prone to damage. Then use a slicing clip to clamp the slide tightly, rotate the screw of the platform mover, and align the part to be observed with the center of the light hole.
(5) Adjust the focal length
Rotate the coarse adjuster counterclockwise with your left hand to slowly raise the stage to a distance of about 5 millimeters between the objective lens and the specimen. Be careful not to observe from the eyepiece while raising the stage. Be sure to watch the stage rise from the right side to avoid excessive rise and damage to the lens or specimen. Then, open both eyes simultaneously and observe with the left eye on the eyepiece. Slowly rotate the coarse adjuster clockwise with the left hand to lower the stage slowly until a clear object image appears in the field of view.
Attention: If the object image is not in the center of the field of view, adjust the slide pusher to adjust it to the center (note that the direction of moving the slide is opposite to the direction of moving the object image in the field of view). If the brightness within the field of view is not suitable, it can be adjusted by adjusting the position of the light collector or the size of the aperture. If the stage descends beyond the working distance (>5.40mm) without seeing the object image when adjusting the focal length, it indicates that the operation has failed and should be restarted. Do not blindly raise the stage in a hurry.
