The difference between a fluorescence microscope and a laser confocal microscope
Different principles
1, fluorescence microscope: ultraviolet light as a light source, used to irradiate the object under examination, so that it emits fluorescence, and then observe the shape of the object and its location under the microscope.
2, laser confocal microscope: in the fluorescence microscope image based on the addition of laser scanning device, the use of ultraviolet light or visible light to stimulate the fluorescent probe.
Different characteristics
1, fluorescence microscope: used to study the absorption of substances in the cell, transport, distribution and localization of chemical substances. Some substances in the cell, such as chlorophyll, can fluoresce after irradiation by ultraviolet light; some other substances themselves can not fluoresce, but when stained with fluorescent dyes or fluorescent antibodies, fluorescence can also be fluorescent by ultraviolet light irradiation.
2、Laser confocal microscope: using computer image processing, so as to get the fluorescence image of the internal microstructure of cells or tissues, as well as in the subcellular level to observe such as Ca2+, pH, membrane potential and other physiological signals and cell morphology changes.
Different applications
1, Fluorescence Microscope: Fluorescence microscope is the basic tool for immunofluorescence cytochemistry. It is composed of light source, filter plate system and optical system and other main components. It is the use of a certain wavelength of light to stimulate the specimen to emit fluorescence, through the objective lens and eyepiece system to magnify the specimen to observe the fluorescence image.
2、Laser confocal microscope: laser scanning confocal microscopy has been used for cell morphology localization, three-dimensional structural reorganization, dynamic changes in the process of research, and to provide quantitative fluorescence determination, quantitative image analysis and other practical means of research, combined with other related biotechnology, in the field of molecular and cellular biology in the field of morphology, physiology, immunology, genetics and so on, has been widely used.
