The difference between fluorescence and confocal imaging
1, Fluorescence microscope
1. Fluorescence microscope uses ultraviolet light as a light source to irradiate the object being inspected, causing it to emit fluorescence, and then observe the shape and position of the object under the microscope. Fluorescence microscopy is used to study the absorption, transport, distribution, and localization of substances within cells. Some substances in cells, such as chlorophyll, can fluoresce when exposed to ultraviolet radiation; There are also some substances that cannot emit fluorescence themselves, but can also emit fluorescence if stained with fluorescent dyes or fluorescent antibodies and irradiated with ultraviolet light. Fluorescence microscopy is one of the tools for qualitative and quantitative research on such substances.
2. Principle of fluorescence microscope:
(A) Light source: The light source emits light of various wavelengths (from ultraviolet to infrared).
(B) Excitation filter light source: Transmitting light of a specific wavelength that can cause fluorescence in the specimen, while blocking light that is useless for exciting fluorescence.
(C) Fluorescent specimens: usually stained with fluorescent pigments.
(D) Blocking filter: It selectively transmits fluorescence by blocking excitation light that is not absorbed by the specimen, and some wavelengths in the fluorescence are also selectively transmitted. A microscope that uses ultraviolet light as a light source to make the illuminated object emit fluorescence. The electron microscope was first assembled by Knorr and Haruska in Berlin, Germany in 1931. This microscope uses high-speed electron beams instead of light beams. Due to the much shorter wavelength of electron flow compared to light waves, the magnification of an electron microscope can reach 800000 times, with a minimum resolution limit of 0.2 nanometers. The scanning electron microscope, which was first used in 1963, allows people to see the tiny structures on the surface of objects.
3. Application scope: Used to enlarge images of small objects. Generally used for observations of biology, medicine, microscopic particles, etc.
