The difference between laser confocal microscope and scanning electron microscope
Both the laser confocal microscope and the scanning electron microscope are point-by-point scanning imaging, and the magnification is adjusted by controlling the scanning driving range. Laser confocal microscopy works by laser scanning and can obtain three-dimensional images. The scanning electron microscope uses various physical signals excited by the finely focused electron beam to scan the surface of the sample to modulate imaging. Only two-dimensional images can be obtained, but three-dimensional images cannot be obtained.
1. The limit resolution is different (the amplified signal source is different)
Laser Confocal: Ultimate Resolution 150nm
Scanning electron microscope: 20nm~0.8nm
2. Different scanning drive methods
Laser confocal: the laser rotating mirror controls the laser scanning range and scanning speed
Scanning Electron Microscope: The electromagnetic coil controls the scanning range and scanning speed of the electron beam
3. Different stereo imaging
Laser confocal: The sample is imaged layer by layer in the Z-axis direction through a nanometer-precision stepping motor, and the software synthesizes the images of each layer set into a clear three-dimensional image
Scanning Electron Microscope: A single frame image has a large depth of field and is a two-dimensional image
4. The scope of application is different
Laser confocal: several times ~ thousands of times
Scanning electron microscope: several times ~ hundreds of thousands of times
5. The working environment is different
Confocal Laser: Can test samples in atmospheric environment
Scanning Electron Microscopy: Testing Samples in a High Vacuum Environment
