The differences between light and electron microscopes are noted.

Mar 09, 2023

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The differences between light and electron microscopes are noted.

 

The difference between an optical microscope and an electron microscope is that an optical microscope can only see certain cell structures, such as cell walls, chloroplasts, stained chromosomes, mitochondria, nuclei, etc. small organelles. In short, the light microscope can see the microscopic structure of cells, and the electron microscope can see the submicroscopic structure.
The main difference is the magnification. Optical microscopes have a magnification limit, and no matter how large the zoom is, the human eye cannot distinguish it. y(min)=0.61*wavelength)/(n*sinu) —— Even if n*sinu is immersed in oil, the maximum is about 1.5, and the rest depends on the size of the wavelength. Therefore, the maximum optical microscope is about 1000 times, and it is useless to zoom in. Electron microscopes use electron beams for imaging, and the wavelength is much smaller than that of visible light, so the minimum resolution distance y(min) is much smaller, and smaller details can be resolved, and the magnification can reach several million.

 

2 Electronic Microscope

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