The fluorescence range of the microscope becomes smaller, and the 40x objective lens cannot see clearly?
1. The neutral gray filter is not completely in place. At this time, you will see that some parts are bright and some are not. The bright part and the non-bright part are roughly the boundaries of an arc;
2. The aperture is closed. At this time, you should see that the center is bright and the edge is dark or there is no fluorescence. If the center of the aperture is not correct, it is not bright in the middle, but a certain place is bright. At this time, you can see The bright part is probably a regular polygon;
3. The center of the mercury lamp is not correct: it may be that the center has not been adjusted properly after replacing the new bulb, or the adjustment rod on the light box has been animated by curious research. At this time, it is difficult to describe the partly bright shape. Yes, but it is basically not the same shape as 1 and 2. You can basically see a more regular and clear boundary in the first 2, but in this case, you can hardly see a regular and clear boundary;
4. The turntable of the excitation block is not turned in place or the beam splitter prism is not pulled in place: this possibility is relatively small, and the resulting image deviation is similar to case 1;
