The knowledge points of 3D ultra-depth-of-field microscope can be easily learned
Since the working distance of the three-dimensional ultra-depth-of-field microscope is very small, everyone must be careful when using the oil lens to prevent damage to the lens on the objective lens and the sample slide during operation.
The process of use usually follows the process of starting from a low-magnification lens, then to a high-power lens, and then to an oil lens. If you are using a high-power lens, then there is no need to switch to a low-power lens and start over, just switch directly to an oil lens.
If the microscope used has the anti-drop function, add a drop of cedar oil to the observed glass slide, move the oil lens down to the oil drop until it stops falling, and then use fine-tuning to make fine adjustments until the object image is clear. Adjust until the focus is adjusted.
The working principle of the 3D ultra-depth-of-field microscope:
1. Refraction and Refractive Index:
Light travels in a straight line between two points in a homogeneous isotropic medium. When it passes through transparent objects with different densities, refraction occurs, which is caused by the different propagation speeds of light in different media. When the light that is not perpendicular to the surface of the transparent object enters the transparent object (such as glass) from the air, the light changes direction at its interface and forms a refraction angle with the normal.
2. Lens performance:
Lenses are the basic optical components that make up the optical system of a three-dimensional ultra-depth-of-field microscope. The objective lens, eyepiece, and condenser are all composed of single or multiple lenses. According to their shapes, they can be divided into two categories: convex lenses (positive lenses) and concave lenses (negative lenses).
When a beam of light parallel to the optical axis passes through a convex lens and intersects at a point, this point is called the "focus point", and the plane passing through the intersection point and perpendicular to the optical axis is called the "focal plane". There are two focal points, the focus in the object space is called "object focus", and the focal plane there is called "object focal plane"; conversely, the focus in image space is called "image focus", and the focal plane there is called "image focal plane".
After light passes through a concave lens, it forms an erected virtual image, while a convex lens forms an erected real image. Real images can appear on the screen, but virtual images cannot.
