A polarizing microscope's two polarizing filters are positioned at a 90° angle from one another to achieve the so-called dark position, in which the field of view is entirely black. If the sample is optically isotropic (a single refractor), the field of view remains dark regardless of how the stage is rotated. This is due to the fact that the polarizer's created linearly polarized light still vibrates in the same direction. The Xie light has zero intensity under Marius' law. The field of view changes if the sample exhibits birefringence.brighter. This is due to the fact that when linearly polarized light from the polarizer penetrates the birefringent body, it creates two linearly polarized lights with different vibration directions (o light and e light). As soon as the two types of light enter the analyzer The brilliant image can be seen in the field of view by passing the analyzer when the e-light does not respect the law of refraction and the polarization direction of the analyzer is not 90°.
Second, the main application of polarized light microscope
1. Biological field
In living organisms, different fibrin structures show marked anisotropy, and using polarized light microscopy, detailed molecular arrangements in these fibers can be obtained. Such as collagen, spinning silk during cell division, etc.
2. Identification of various biological and non-biological materials
Such as starch property identification, drug composition identification, fiber, liquid crystal, DNA crystal, etc.
3. Medical analysis
Such as stones, uric acid crystal detection, arthritis, etc.
4. Geological and Mineral Analysis
In addition to common biomedical applications, polarized light microscopes can also be used for polarized light detection of various minerals and crystals, and are widely used in petroleum, mining and body industries. LED lighting and special filters can be used in quality control and industry analysis.
