The steps and precautions for using a stereo microscope
Stereoscopic microscope is a small microscope with macroscopic magnification, which can be used for observation, photography, analysis, etc. There are also excellent research grade stereomicroscopes (with higher magnification and better objective), generally magnifying around 6-200 times. Stereoscopic microscopy is generally only used for stereotypical analysis, and for qualitative measurements, it is not very accurate. It is necessary to know the determined body magnification ratio and cooperate with software or an eyepiece measuring ruler to complete it.
The specific steps for using a Leica microscope are as follows:
(1) After installing the microscope, ensure that the power supply voltage is consistent with the rated voltage of the stereo microscope before plugging in the power plug, turning on the power switch, and selecting the lighting method;
(2) Based on the observed specimen, select a table (for transparent specimens, use ground glass table; for opaque specimens, use black and white table), place it in the hole of the base table, and lock it tightly;
(3) Loosen the fastening screws on the focusing slide and adjust the height of the mirror body to a working distance that is roughly consistent with the selected objective lens magnification. After adjustment, lock the bracket, place the safety ring against the focusing bracket and lock it tightly;
(4) After installing the eyepiece, first loosen the screw on the eyepiece cylinder, and then tighten the screw (when placing the eyepiece into the stereo microscope eyepiece cylinder, be particularly careful not to touch the surface of the lens with your hands);
(5) Adjust the pupil distance. When the user observes the field of view through two eyepieces and it is not a circular field of view, the prism box should be pulled to change the distance between the exit pupils of the eyepiece tube, so that a completely overlapping circular field of view can be observed (indicating that the pupil distance has been adjusted);
(6) Observe the specimen (focus the specimen). First, adjust the visibility circle on the left eyeglass tube to the 0 mark position. Normally, first observe from the right eyepiece tube (i.e. fixed eyepiece tube), turn the zoom tube (when equipped with a zoom device) to the * high magnification position, rotate the focusing handwheel to focus the specimen until the image of the specimen is clear, then turn the zoom tube to the * low magnification position. At this time, observe with the left eyepiece tube. If it is not clear, adjust the viewing circle along the axial direction of the lens tube until the image of the specimen is clear, and then observe its focusing effect with both eyes;
(7) At the end of the observation, turn off the power, remove the specimen, and tightly cover the microscope with a dust cover.
