The steps to use the phase contrast microscope are as follows
① Select a suitable phase contrast objective lens according to the nature and requirements of the specimen to be inspected.
②Put the specimen slide on the stage and adjust the center of the optical axis.
③Use the coaxial adjustment telescope, adjust the ring diaphragm to completely overlap with the conjugate surface ring on the phase plate, and then replace the eyepiece. During the observation process, every time the multiple of the objective lens is changed, it is necessary to re-adjust the annular diaphragm to coincide with the ring of the conjugate surface of the phase plate.
④ Add a green filter and observe according to the operating steps of an ordinary optical microscope.
