Three calibration methods for biological microscopes
Biological microscopy is widely used in fields such as histology, cytology, parasitology, microbiology, oncology, immunology, genetic engineering, and botany. Resolution is an important indicator for observing the effect Z in order to obtain clear and bright ideal images and satisfactory microscopy results. From a physical perspective, the resolution of biological microscopes is limited by the interference phenomenon of light and the performance of the objective lens used. Therefore, various optical technical parameters of biological microscopes should be calibrated according to standards, and quality control should be implemented for items that require accurate measurement values during the calibration process to ensure the accuracy and reliability of measurement results.
Calibration methods for biological microscopes
1. In terms of appearance
Biological microscopes should be labeled with information such as name, model, serial number, manufacturer, etc. The imaging of the optical system should be clear throughout the entire field of view, and there should be no shadows or reflection spots that affect observation within the field of view. The clear area of imaging should be concentric with the field of view.
The movement of each part of the biological microscope should be flexible and stable, without any jamming or jumping phenomenon, and the illumination within the field of view should be uniform. The spectral colors of the left and right systems of a binocular microscope should be basically consistent, without significant differences in brightness.
2. Fine tune the spacing between handwheel markings
The fine-tuning handwheel should be flexible and stable, with moderate elasticity. The spacing between the fine tuned handwheel markings should be measured at the beginning, middle, and end positions of the handwheel. Before measurement, fix the indicator on the biological microscope with a dedicated fixture to ensure that it is securely fastened to the microscope. When fixing the indicator, ensure that the measuring head is in vertical contact with the stage. Adjust the focusing handwheel of the biological microscope to zero the reading of the indicator. Rotate the focusing handwheel by 10 scales, read the indicated value, and use 1/10 of the indicated value as the measurement result. Take the average measurement at the beginning, middle, and end positions as the calibration result.
3. Magnification error of biological microscope objective lens
Adjust the light source to the appropriate position, install the eyepiece with a graduated ruler on one of the sleeves of the biological microscope, place the standard glass ruler on the stage, adjust the eyepiece and standard glass ruler so that the reticle line is parallel to the standard glass ruler line to avoid measurement errors. Adjust the stage so that the zero position of the glass ruler is aligned with one end of the micrometer, and then read directly.
